You can find the biology homework attachedYou can find the biology homework attachedYou can find the biology homework attached1. Clathrin and COP-coated vesicles mediate transport of proteins inside the cell. Indicate
what you would observe if you omitted the Rab proteins and explain which vesicular
transport pathway will be affected:
2. Predict the final location of a protein that has been engineered to include an ER signal
sequence, a mannose-6-phosphate tag, and a nuclear localization signal. Justify your
3. Compare and contrast the movement of proteins into the nucleus versus the ER lumen.
4. Determine the mature topology for each of the 3 different polypeptides given below.
Please be sure to indicate removal of any portion of the polypeptide if it is not retained in
the mature protein.
5. The membrane-spanning region of many transmembrane proteins is frequently an α helix
comprising of (fill in the blank) ——————— amino acid residues.
These α helices can be identified by means of a hydropathy plot (see below), which
predicts potential membrane-spanning segments in a polypeptide chain. For example, a
helix of valine residues in a protein would generate a score of 0.54, i.e. values close to, and
above, zero represent non-polar sequences.
Based on the following hydropathy plot and given that a transmembrane segment is about
20 amino acid long, how many transmembrane domains would you expect this protein to
have? Label them on the plot below and be sure to provide an explanation for how you
reached that conclusion.
6. You are trying to identify a signal sequence “Retention Signal Sequence; RSS) in protein
X that ensures that proteins that are destined to stay in the ER lumen are shipped back to
ER in case they escaped to Golgi.
To localize RSS, you created a set of sequence deletion at N-and C- terminus of Protein
X followed by immunofluorescence to localize it. Please note that a Box represents a
a) Which amino acid residues are necessary for Protein X retention in ER lumen?
b) How would you show that the peptide sequence you identified in part a) is sufficient
to cause retention of any protein in ER lumen?
7. One important feature of any intracellular signaling pathway is its ability to be turned off.
Consider the following pathway:
a) Which residues located in the intracellular domain are phosphorylated?
b) Provide 2 ways by which this pathway can be turned off.
c) Assume that molecule 2 is Grb2. Draw on the figure Ras in its active form and the proteins
that mediate its activation.
d) Name 3 proteins that are activated downstream of active Ras.
e) In a series of experiments, you introduce into your cells a gene that codes for an RTK
lacking its intracellular domain. Assume that the cell is still expressing its own normal form
of the receptor, what would be the consequence of introducing this mutant gene of RTK?
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