I need help with my bio homework that contains just 5 questionsattachedattachedI need help with my bio homework that contains just 5 questions1.
Clathrin and COP-coated vesicles mediate transport of proteins inside the cell. Indicate
what you would observe if you omitted the Rab proteins and explain which vesicular
transport pathway will be affected:
2. Predict the final location of a protein that has been engineered to include an ER signal
sequence, a mannose-6-phosphate tag, and a nuclear localization signal. Justify your
3. Compare and contrast the movement of proteins into the nucleus versus the ER lumen.
4. Determine the mature topology for each of the 3 different polypeptides given below.
Please be sure to indicate removal of any portion of the polypeptide if it is not retained in
the mature protein.
5. The membrane-spanning region of many transmembrane proteins is frequently an α helix
comprising of (fill in the blank) ——————— amino acid residues.
These α helices can be identified by means of a hydropathy plot (see below), which
predicts potential membrane-spanning segments in a polypeptide chain. For example, a
helix of valine residues in a protein would generate a score of 0.54, i.e. values close to, and
above, zero represent non-polar sequences.
Based on the following hydropathy plot and given that a transmembrane segment is about
20 amino acid long, how many transmembrane domains would you expect this protein to
have? Label them on the plot below and be sure to provide an explanation for how you
reached that conclusion.
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